Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.084045 |
Chromosome: | chromosome_11 |
Location: | 400129 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467580 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCTTCTGCTGCTCGTCAAGCTTTGCAAGC |
Internal bar code: | CGGACGAGCTGAAAGCAACAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 531 |
LEAP-Seq percent confirming: | 69.3645 |
LEAP-Seq n confirming: | 1157 |
LEAP-Seq n nonconfirming: | 511 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCTTGCTGTCCAGCTCTTG |
Suggested primer 2: | AGGTACGCCTGGTGTGTAGG |