| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.084080 |
| Chromosome: | chromosome 1 |
| Location: | 4900482 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034000 | IPB1 | Importin beta; (1 of 1) K14293 - importin subunit beta-1 (KPNB1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGGGTGGTGGCGTCGGGGGTGGAGGC |
| Internal bar code: | GCATCGACTTGGGTCGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 91.4186 |
| LEAP-Seq n confirming: | 522 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGGTGTCCATCATCCT |
| Suggested primer 2: | CGAATCCAAGTCCACACCTT |