| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084107 |
| Chromosome: | chromosome 1 |
| Location: | 2086184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011150 | (1 of 2) IPR000104//IPR011598 - Antifreeze protein, type I // Myc-type, basic helix-loop-helix (bHLH) domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGAGATCCTGGTATCAGCGCCTCATCA |
| Internal bar code: | CTCACTTCTGAACACTGCCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 95.4774 |
| LEAP-Seq n confirming: | 570 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCAGTTTCGCGCATTAT |
| Suggested primer 2: | ACCGCCTAGTAAACTCCCGT |