Insertion junction: LMJ.RY0402.084116_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.084116
Chromosome:chromosome 15
Location:563924
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre15.g637249 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTGTGCGTGCTGGTAATGACAACCGCT
Internal bar code:TGGATTTCGGCGCACTCCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:16
LEAP-Seq percent confirming:98.9501
LEAP-Seq n confirming:1131
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AATCGTAACCCTTGACGCAC
Suggested primer 2:AACGGTTGAGTGCCGTATTC