| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.084142 |
| Chromosome: | scaffold 35 |
| Location: | 29830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre35.g759447 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGGCGGCGGAACGTTTGTGAGCAACA |
| Internal bar code: | ATGACCTGGTGGACCCATAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 70.6383 |
| LEAP-Seq n confirming: | 332 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAACGTGTGGTGGATGTG |
| Suggested primer 2: | ACACTTGTCCGTCACCATCA |