| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084145 |
| Chromosome: | chromosome 1 |
| Location: | 4070562 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026650 | (1 of 1) PTHR23293//PTHR23293:SF5 - FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE // PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTCCGCGGCATGCCTGCCTCGGGGCTG |
| Internal bar code: | ATGGCCCGATTAGTACCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.6788 |
| LEAP-Seq n confirming: | 5586 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTCGTAATGCCTAATGT |
| Suggested primer 2: | CATACACACGGTATGCGAGG |