| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084166 |
| Chromosome: | chromosome 9 |
| Location: | 1791574 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396200 | CSV4 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGGATGAAACGGGTGGCTCGTCCCGTG |
| Internal bar code: | TGAGAAGGGCAATGCCGGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 98.765 |
| LEAP-Seq n confirming: | 2959 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATACGTTGCCGCTTACCG |
| Suggested primer 2: | AGTAGCGCGATCGACTGATT |