Insertion junction: LMJ.RY0402.084173_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.084173
Chromosome:chromosome 9
Location:2650915
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g390000 SRH17 SNF2-related DNA/RNA helicase; (1 of 2) K11654 - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 [EC:3.6.4.-] (SMARCA5, SNF2H, ISWI) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCGGTGCGTGCTGCGCTGGGCAAGCTGC
Internal bar code:GGGCGCTAGTTCCCGCACCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:99.8299
LEAP-Seq n confirming:587
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACACCCGTCATCTTCT
Suggested primer 2:CGATACCTAGCACCTGCTCC