| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084195 |
| Chromosome: | chromosome 7 |
| Location: | 4386013 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCGTCTGCTCTCGCGGAAGCATGATG |
| Internal bar code: | CCGCTAAGCCCGCCGACGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 99.7924 |
| LEAP-Seq n confirming: | 13937 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCCAGGCTCAGTTTAG |
| Suggested primer 2: | CGGTAAGGCCTTTCACACAT |