Insertion junction: LMJ.RY0402.084199_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.084199
Chromosome:chromosome 9
Location:1756444
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396451 GOX15 (1 of 1) IPR011043//IPR014756//IPR015202 - Galactose oxidase/kelch, beta-propeller // Immunoglobulin E-set // Domain of unknown function DUF1929; Glyoxal oxidase 15 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGCCCCATGTCCCCGTCATTGGTGTTT
Internal bar code:CCCTGGTTTGTGCGCACGCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:573
LEAP-Seq percent confirming:99.7252
LEAP-Seq n confirming:2177
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGTGAACATGCCGTCCCT
Suggested primer 2:TTCAGTAGTTGGCAATCCCC