Insertion junction: LMJ.RY0402.084204_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.084204
Chromosome:chromosome 12
Location:4079818
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g517750 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGCTATGTTTCGCGCACGAGGGCATGC
Internal bar code:AAGGTGAGTAAGACCTGCGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:562
LEAP-Seq percent confirming:99.1189
LEAP-Seq n confirming:225
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCCACTTCTTCACAACAT
Suggested primer 2:AGGTGAACCAACATTCTCCG