Insertion junction: LMJ.RY0402.084219_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.084219
Chromosome:chromosome 10
Location:3359537
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g443950 (1 of 2) PF04564//PF05049 - U-box domain (U-box) // Interferon-inducible GTPase (IIGP) (IIGP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTTTCCCCGGGCAGCGCGCCGTTTTGCG
Internal bar code:TACAACGGCATTCGCCCGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:595
LEAP-Seq percent confirming:96.8958
LEAP-Seq n confirming:437
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCACGAAGTACTCGACA
Suggested primer 2:AGTACAGGCAGATAGGCGGA