| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084289 |
| Chromosome: | chromosome 17 |
| Location: | 19905 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696250 | (1 of 2) K03260 - translation initiation factor 4G (EIF4G) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTGCGCCTCGCGGTGCACGTCCTCAAT |
| Internal bar code: | CAGCCTGTCTTGGCGATCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 18 |
| LEAP-Seq percent confirming: | 97.4395 |
| LEAP-Seq n confirming: | 685 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCTTGGCAATTGGACAT |
| Suggested primer 2: | CCCCTACTACTTGCAGCTCG |