| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084294 |
| Chromosome: | chromosome_1 |
| Location: | 3953166 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025750 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTATCCTAATGCAGAGTTATGCGTGCTTC |
| Internal bar code: | AGCCATTGTTTGGCAGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 912 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACAGTAACTTGGTGGGG |
| Suggested primer 2: | TGAGGAGGGCAAGGCTAGTA |