Insertion junction: LMJ.RY0402.084323_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.084323
Chromosome:chromosome 8
Location:870039
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g361250 (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGTAGTCATGCCTGAACACCCCGCTCC
Internal bar code:TGCCTTTCCTAAGTCTACTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:91
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCTCATACACATGCCAG
Suggested primer 2:TCACACACGGCTACTTCCTG