| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.084382 |
| Chromosome: | chromosome 5 |
| Location: | 2239455 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGTGTCTGTGACTCTGCCCCACGCACC |
| Internal bar code: | CGTAGCGTGGGAGAACTACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 98.21 |
| LEAP-Seq n confirming: | 3676 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGGAAAGATCCTCACCA |
| Suggested primer 2: | AATGACCGAACAGATCGAGG |