| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084393 |
| Chromosome: | chromosome 17 |
| Location: | 3120569 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACTGCGGCACGCGACGCCCACTTACGC |
| Internal bar code: | TGGCCCTGCCGACAGCATGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 98.2739 |
| LEAP-Seq n confirming: | 1765 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGTAGCGGATCAACACC |
| Suggested primer 2: | GCACGTCAATGTAACCGTTG |