| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084396 |
| Chromosome: | chromosome 10 |
| Location: | 2131350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCATTACTGCCGGCAGCTCTGCACGCCA |
| Internal bar code: | CCGTACGGTCTGACTCAGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 79.9065 |
| LEAP-Seq n confirming: | 513 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTAGCGCACAAATCCC |
| Suggested primer 2: | TTGTGGAGGACGATAGACCC |