Insertion junction: LMJ.RY0402.084398_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084398
Chromosome:chromosome 9
Location:6517337
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATTCGTGTTTAGCTGTTTGAACGCTTC
Internal bar code:AGGCCCCTCCTAAGGACGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:97.983
LEAP-Seq n confirming:1846
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGATGCATAGACGTGCG
Suggested primer 2:ACTAATACGGGTGTGGCAGC