| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084408 |
| Chromosome: | chromosome 3 |
| Location: | 4260336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174350 | PWR2 | (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTTCCAGTCGCGAGCAGCGAACTTGTT |
| Internal bar code: | TACCGGTGGGCGACGCCACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 99.8017 |
| LEAP-Seq n confirming: | 6543 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTGTTGTGGGCCTAAT |
| Suggested primer 2: | ATGACGACGTGATTGCATGT |