| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084410 |
| Chromosome: | chromosome_7 |
| Location: | 2367711 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g328350 | FAP207 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTTGGCTGAGAGCGGAGGTCTGAAACGTG |
| Internal bar code: | ATTATAGCGCCATAGCGTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 99.2571 |
| LEAP-Seq n confirming: | 668 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACCTGACAACAAACGG |
| Suggested primer 2: | TGTCGCACACAATCACACTG |