| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084413 |
| Chromosome: | chromosome 15 |
| Location: | 466793 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636600 | FAP391 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1); Flagellar Associated Leucine Rich Repeat Protein 391 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACACGCGGGTCCACACACGCACACACG |
| Internal bar code: | AAACGGCATTGTTATTGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 99.5951 |
| LEAP-Seq n confirming: | 246 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCTTAACGTCCGCATC |
| Suggested primer 2: | TAATTAACGTTTCCAGGCCG |