Insertion junction: LMJ.RY0402.084424_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.084424
Chromosome:chromosome 11
Location:2273954
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTCTGCATTGCAAATAGGTCAGGCAGC
Internal bar code:GTCACAGCTGGCGAGCGTGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:444
LEAP-Seq percent confirming:51.9573
LEAP-Seq n confirming:107760
LEAP-Seq n nonconfirming:99641
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCTAGGTTCCCAGAGTG
Suggested primer 2:ATGTCGCCTGACCTATTTGC