| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084433 |
| Chromosome: | chromosome 11 |
| Location: | 1129367 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467680 | (1 of 13) 3.1.13.4 - Poly(A)-specific ribonuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGGTTGCGAGTGGGAACAGACTCCAA |
| Internal bar code: | GGGGTCGGATCTTTGCGACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 845 |
| LEAP-Seq percent confirming: | 99.6408 |
| LEAP-Seq n confirming: | 17753 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGCTTGAAAATGATTGA |
| Suggested primer 2: | CCTTCACCTCCATCCACAAC |