| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084443 |
| Chromosome: | chromosome 7 |
| Location: | 4133012 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342750 | (1 of 1) K18106 - D-galacturonate reductase (GAAA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCTTGGGCATGTGGCCATCTGCCCCT |
| Internal bar code: | GAGTGCAGACACAAACGCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAATGAAGTCACGCAGCC |
| Suggested primer 2: | TTGACAGCTCTGCACAATCC |