Insertion junction: LMJ.RY0402.084458_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084458
Chromosome:chromosome 14
Location:2557955
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g625625 FTSH11 FtsH-like membrane ATPase/metalloprotease; (1 of 2) IPR000642//IPR003593//IPR005936//IPR011704//IPR027417 - Peptidase M41 // AAA+ ATPase domain // Peptidase, FtsH // ATPase, dynein-related, AAA domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACCCAGCGCTCCACCAGCGCTTCCTAC
Internal bar code:GGTGGCGCAGTGCCCGACATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:82.7432
LEAP-Seq n confirming:5749
LEAP-Seq n nonconfirming:1199
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAATGAAATCCCCTCGAC
Suggested primer 2:CTCTGCTGGACAAGGAGACC