| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084462 |
| Chromosome: | chromosome_7 |
| Location: | 2498168 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g329500 | SUB1 | Secreted protease and protease inhibitor | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGCAGCGTCAGGCCAGGCCTTGCTGCCAC |
| Internal bar code: | GAAGCCGAGCCTGACCGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 99.7543 |
| LEAP-Seq n confirming: | 3654 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCGCCCTGTTCACAGAC |
| Suggested primer 2: | GAGTCACCGCCGTTATTGTT |