| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084476 |
| Chromosome: | chromosome 12 |
| Location: | 4401428 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCGCAGTAGCAGTGGGGGTAGTGACC |
| Internal bar code: | TATGTCAACACTTGTTTTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 99.1477 |
| LEAP-Seq n confirming: | 4537 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAGTCTCCGTCTTCTGG |
| Suggested primer 2: | ACAGCACTGGGTTGATGGAT |