Insertion junction: LMJ.RY0402.084489_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.084489
Chromosome:chromosome 9
Location:1108286
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g400312 (1 of 5) IPR001680//IPR011047//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCTCCTGCCCGAGTTTGGTGACTGGGG
Internal bar code:TCGGACGTCGACAGAGTTGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:307
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGTCCAGCCTTTGCTGC
Suggested primer 2:CCGGTGTGAAGCATACCTTT