| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084499 |
| Chromosome: | chromosome 10 |
| Location: | 3699329 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446100 | TRX6,TRXY,TRXY1 | Thioredoxin y, chloroplastic; (1 of 1) PTHR10438:SF280 - THIOREDOXIN Y1, CHLOROPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTTCGTCACCATCGCCCACATATGACA |
| Internal bar code: | GCTATTACAGTGTGGGGAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.661 |
| LEAP-Seq n confirming: | 294 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGACACGATCAAGGAA |
| Suggested primer 2: | GTTGATTACGCCGGAACAGT |