Insertion junction: LMJ.RY0402.084501_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.084501
Chromosome:chromosome 6
Location:7243674
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g298200 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGAGTCGACAGGGTCGCATCGCTGCTCT
Internal bar code:GGTGTTTCTCATCGTGGTACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:99.5162
LEAP-Seq n confirming:6171
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCGCTGACACATACATC
Suggested primer 2:ACGTCTAGCTTCTCCAGCCA