| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084508 |
| Chromosome: | scaffold 19 |
| Location: | 89197 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGGTGCGAGGGCCCCGCTGGCGCCCA |
| Internal bar code: | AACGGTGTGTCGTGCGGGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 82.0648 |
| LEAP-Seq n confirming: | 50126 |
| LEAP-Seq n nonconfirming: | 10955 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGGGGTTCTGGAAAAA |
| Suggested primer 2: | AGTGGGATGCGTGTTAGGTC |