Insertion junction: LMJ.RY0402.084510_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.084510
Chromosome:chromosome 3
Location:7875457
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g202550 ELG11 Exostosin-like glycosyltransferase 11; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAATGGTCCCGCGCCCCGTAACCCACCC
Internal bar code:GGCTATGCGCTGATTCAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:631
LEAP-Seq percent confirming:99.9216
LEAP-Seq n confirming:2550
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATACACGCAGAGCTACA
Suggested primer 2:CGTACCTTAAAAGGCCACCA