| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084521 |
| Chromosome: | chromosome_2 |
| Location: | 8414691 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141606 | DHC9 | Flagellar inner arm dynein heavy chain, dynein c (monomeric) | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATTCATACGCTCCACTGAATTGCCAGTTT |
| Internal bar code: | TAAGTCTCGCGCCCACGTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.0054 |
| LEAP-Seq n confirming: | 1095 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGGTAGGAGGGATTGC |
| Suggested primer 2: | TGACACGCTCCAACTCACTC |