Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.084521 |
Chromosome: | chromosome_2 |
Location: | 8414693 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g141606 | DHC9 | Flagellar inner arm dynein heavy chain, dynein c (monomeric) | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGGGGCCAGTGCCATGGCCTTCAAGGGT |
Internal bar code: | GCTAAGCACGATCTCGCTCTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 132 |
LEAP-Seq percent confirming: | 78.6885 |
LEAP-Seq n confirming: | 48 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAGAGGTAGGAGGGATTGC |
Suggested primer 2: | TGACACGCTCCAACTCACTC |