| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084524 |
| Chromosome: | chromosome 12 |
| Location: | 8614789 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547100 | CGL2,SMM43 | (1 of 2) PTHR10108//PTHR10108:SF788 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; putative methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATGCTGATTACGGCTGATAGCCTGATA |
| Internal bar code: | AGACTGGCAGTTTAGAATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 45.9459 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGAAAGCAAAGAAGCA |
| Suggested primer 2: | TCACCTACAGCAAGGTGTGC |