| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084527 |
| Chromosome: | chromosome 9 |
| Location: | 1865023 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395646 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCGTCGCACTCAAGCAACGTGTGTCTT |
| Internal bar code: | GGAATCGGGGGCTTACCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 99.039 |
| LEAP-Seq n confirming: | 3298 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCTCCTTGTCCCACTC |
| Suggested primer 2: | GGCTTCAACAACTAGCCAGC |