| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084543 |
| Chromosome: | chromosome 17 |
| Location: | 3526870 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725250 | (1 of 39) IPR009003 - Peptidase S1, PA clan | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGGTTTATGCTGTACCCGCCATCACGA |
| Internal bar code: | TGGCGCAGCAAGTGGGTAAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 98.1026 |
| LEAP-Seq n confirming: | 1396 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCTGGTGGCCTGTGTC |
| Suggested primer 2: | TTCTCTCCCAGTTGGCTTGT |