Insertion junction: LMJ.RY0402.084546_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084546
Chromosome:chromosome 6
Location:4862319
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278700 Conserved Ankyrin-Repeat Protein; (1 of 1) PTHR24193:SF69 - IQ MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN LOC642574 HOMOLOG-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTGAGGATGATCGACGTCGGATCGGAGG
Internal bar code:CTTTGGCATCGCGGCGATCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:756
LEAP-Seq percent confirming:99.2269
LEAP-Seq n confirming:3337
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCCTGCTACCCACAGGT
Suggested primer 2:ATATGGACATGGTGGGTGCT