| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084547 |
| Chromosome: | chromosome 1 |
| Location: | 3132949 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019500 | (1 of 2) PTHR22847//PTHR22847:SF431 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGTTGCGGTGTACGCGGGAGAGGCAT |
| Internal bar code: | GCGTCCTCGCTCTTGTAGCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.5574 |
| LEAP-Seq n confirming: | 6523 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCGAGTGCTGTTGAAGA |
| Suggested primer 2: | GACATGCAGCTTCGACAAGA |