| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084549 |
| Chromosome: | chromosome 12 |
| Location: | 5160547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527300 | RFC5 | DNA replication factor C complex subunit 5; (1 of 1) PTHR11669//PTHR11669:SF9 - REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT // REPLICATION FACTOR C SUBUNIT 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGAAGGGAACGGAGGTGCAGGGCTTG |
| Internal bar code: | CGAAAAACTGAGGACATTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 99.6903 |
| LEAP-Seq n confirming: | 9980 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGAGCGTTTCTGTGCAA |
| Suggested primer 2: | TCTGGGCTTGGAACTGACTT |