| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084584 |
| Chromosome: | chromosome 3 |
| Location: | 3778978 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170150 | (1 of 2) PTHR10857//PTHR10857:SF0 - COPINE // PROTEIN GEM-4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCTTTCCAGTGGCCGACGCAATCCATG |
| Internal bar code: | GGCATAGGCTTTTATGTATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 99.2 |
| LEAP-Seq n confirming: | 496 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGACACTGCACGTTGTTG |
| Suggested primer 2: | TCTGGTCCAATCCTCACACA |