Insertion junction: LMJ.RY0402.084595_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084595
Chromosome:chromosome 3
Location:7885658
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g202500 (1 of 1) PF04836//PF05004 - Interferon-related protein conserved region (IFRD_C) // Interferon-related developmental regulator (IFRD) (IFRD) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACTTTGACGTGATGTGGGCGATTGAAG
Internal bar code:TTGGGAGACGTGAGGGGGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:99.3276
LEAP-Seq n confirming:1034
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCAACCCTCTTCCTGCT
Suggested primer 2:CAACGCAGCTTGGATACTGA