| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084614 |
| Chromosome: | chromosome 10 |
| Location: | 5974787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462400 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGGGGCGTGGGCGGGCTTTGGCGGAG |
| Internal bar code: | TGGTGCAATCCGAGGGCGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 9.96126 |
| LEAP-Seq n confirming: | 180 |
| LEAP-Seq n nonconfirming: | 1627 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATAGGACCAGCACCAGA |
| Suggested primer 2: | AGTTTCCAAAACGCATACCG |