| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084614 |
| Chromosome: | chromosome 10 |
| Location: | 5974794 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462400 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCATCTTCCTGGTTTCTCAGGCCGCCG |
| Internal bar code: | GCGCGTTACCGACGCTACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 96.8504 |
| LEAP-Seq n confirming: | 246 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATAGGACCAGCACCAGA |
| Suggested primer 2: | AGTTTCCAAAACGCATACCG |