| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084634 |
| Chromosome: | chromosome 12 |
| Location: | 69894 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483800 | CPY | Serine carboxypeptidase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCGGCGCGTGCTATGATGCATGCACAG |
| Internal bar code: | CCCAGAGAGACAGGAGCTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.4701 |
| LEAP-Seq n confirming: | 5631 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGTACCGATCCACACTG |
| Suggested primer 2: | CAAGGAGGTCACAGCTCTCC |