| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084653 |
| Chromosome: | chromosome 9 |
| Location: | 7093620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411666 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCGCAATTCATTGGCTAATTGTTCGCT |
| Internal bar code: | GAAATATGGACAGCTATTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.7219 |
| LEAP-Seq n confirming: | 11115 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATGCGTACACCATCTC |
| Suggested primer 2: | AAAGGTGCGCACTTCTGTTT |