| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.084691 |
| Chromosome: | chromosome_14 |
| Location: | 1581714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g618750 | FAP246 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATGGGAATGAGAGGGTTTCGCACTTGTCT |
| Internal bar code: | AGGGGCTACGCGAAGGTGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 875 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTGGGTGGAAGTTGTT |
| Suggested primer 2: | TGAGCAAACCACGTAACTGC |