Insertion junction: LMJ.RY0402.084705_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.084705
Chromosome:chromosome 1
Location:3055339
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g018850 EEP2 (1 of 4) K12603 - CCR4-NOT transcription complex subunit 6 (CNOT6, CCR4); Exonuclease-Endonuclease-Phosphatase superfamily protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGGCGGTCCGGCGTGATAAGTAAAGGG
Internal bar code:GACGGTGGCAGATTAGATCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:667
LEAP-Seq percent confirming:99.469
LEAP-Seq n confirming:562
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAGCATCAGCAGGTGACA
Suggested primer 2:TCTGGACTACGTGTGGCTCA