Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.084705 |
Chromosome: | chromosome 1 |
Location: | 3055339 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g018850 | EEP2 | (1 of 4) K12603 - CCR4-NOT transcription complex subunit 6 (CNOT6, CCR4); Exonuclease-Endonuclease-Phosphatase superfamily protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGCGGTCCGGCGTGATAAGTAAAGGG |
Internal bar code: | GACGGTGGCAGATTAGATCAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 667 |
LEAP-Seq percent confirming: | 99.469 |
LEAP-Seq n confirming: | 562 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTAGCATCAGCAGGTGACA |
Suggested primer 2: | TCTGGACTACGTGTGGCTCA |