| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084723 |
| Chromosome: | chromosome 12 |
| Location: | 8011860 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551850 | NFR1 | (1 of 1) 1.5.1.30 - Flavin reductase (NADPH) / Riboflavine mononucleotide reductase; NADH-dependent flavin reductase/cytochrome P450, CYP102 superfamily | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAACCCCCGCCACGAATGGTACTGCAA |
| Internal bar code: | ACGGTGCTTTTCGTGGATGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.5656 |
| LEAP-Seq n confirming: | 1146 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGAGTGAAAACGAGAG |
| Suggested primer 2: | TGGTTATCACATTCGCCTCA |